53 research outputs found
Direct characterization of circulating DNA in blood plasma using ÎĽLAS technology
Circulating cell-free DNA (cfDNA) is a powerful cancer biomarker for establishing targeted therapies or monitoring patients' treatment. However, current cfDNA characterization is severely limited by its low concentration, requiring the extensive use of amplification techniques. Here we report that the ÎĽLAS technology allows us to quantitatively characterize the size distribution of purified cfDNA in a few minutes, even when its concentration is as low as 1 pg/ÎĽL. Moreover, we show that DNA profiles can be directly measured in blood plasma with a minimal conditioning process to speed up considerably speed up the cfDNA analytical chain
ssDNA degradation along capillary electrophoresis process using a Tris buffer
Tris-Acetate buffer is currently used in the selection and the characterization of ssDNA by capillary electrophoresis (CE). By applying high voltage, the migration of ionic species into the capillary generates a current that induces water electrolysis. This phenomenon is followed by the modification of the pH and the production of Tris derivatives. By injecting ten times by capillary electrophoresis ssDNA (50 nM), the whole oligonucleotide was degraded. In this paper, we will show that the Tris buffer in the running vials is modified along the electrophoretic process by electrochemical reactions. We also observed that the composition of the metal ions changes in the running buffer vials. This phenomenon, never described in CE, is important for fluorescent ssDNA analysis using Tris buffer. The oligonucleotides are degraded by electrochemically synthesized species (present in the running Tris vials) until it disappears, even if the separation buffer in the capillary is clean. To address these issues, we propose to use a sodium phosphate buffer that we demonstrate to be electrochemically inactive
French Roadmap for complex Systems 2008-2009
This second issue of the French Complex Systems Roadmap is the outcome of the
Entretiens de Cargese 2008, an interdisciplinary brainstorming session
organized over one week in 2008, jointly by RNSC, ISC-PIF and IXXI. It
capitalizes on the first roadmap and gathers contributions of more than 70
scientists from major French institutions. The aim of this roadmap is to foster
the coordination of the complex systems community on focused topics and
questions, as well as to present contributions and challenges in the complex
systems sciences and complexity science to the public, political and industrial
spheres
Modelisation de l'evolution nycthemerale de l'oxygene dissous en etang
SIGLEINIST T 75372 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc
Estimating the parameters of dissolved oxygen dynamics in shallow ponds
International audienc
Mobidyc, a generic multi-agents simulator for modeling populations dynamics
International audienc
Restoration of lake Geneva : Expected versus observed responses of phytoplankton to decrease in phosphorus
International audienc
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